Adding compounds. Purina TestDiet® can add compounds to virtually any diet at no extra cost, because we make all diets fresh to your order within 7-10 business days.
Be particularly mindful of correctly determining dosage: it is important to determine approximately how much your animals ingest, not how much they consume. Typically animals spill part of their food, some, such as mice, quite a large percentage (10%-30%+); dosage should be based on the amount actually eaten by the animal. We suggest that a short (few day) informal food intake survey be conducted of a representative sample of your animals, accounting for the amount of food leaving the hopper reduced by the amount spilled in the tray below.
Then the dosage percentage should e based on the ingestion amount. For example, a mouse may, with spillage and hoarding, "consume" (use up) 5 grams per day, but actually only ingest 4 grams. If the dosage is based on a "5gram/day" assumption, the animals will only receive about 80% of the intended dosage; if based on a "4gram/day" assumption, they will receive the full amount of the intended dosage.
Calculating the Concentration of Compound in Diet |
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To achieve your desired level of dosing, determine these variables and apply them to either one of two equations.
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Typical Examples
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Mouse |
Rat |
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First, Determine Daily Dose - DD |
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mg of Compound per kg of body weight - CK |
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10 mg |
10 mg |
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Body Weight of animal (gm) - BW |
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35 gm |
300 gm |
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CK(mg) x BW(gm) x .001 = DD |
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Daily Dose of Compound (mg) - DD |
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0.35 mg |
3 mg |
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Second, Determine Concentration in Diet - CD |
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Daily Dose of Compound (mg) - DD |
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0.35 mg |
3 mg |
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Daily Food Intake (gm) - FI |
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3.5 gm |
20 gm |
By applying one of two equations - |
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Either to determine mg per kg diet / ppm: |
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(CKmg x BWgm) / FIgm = CDmg per kg of diet = ppm |
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100 |
150 |
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Or to determine percentage of diet: |
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{(CKmg x BWgm) / FIgm}/10,000 = CD% of diet |
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0.01% |
0.015% |
See: